A Simple, Precise, accurate, sensitive and robust method was developed for simultaneous estimation of Levosulpiride (LS) and Rabeprazole sodium (RS) from their combined dosage form using reverse phase High Performance Liquid chromatography (RP-HPLC) equipped with UV-visible detector. The developed method was validated as per ICH Q2A (R1) guidelines. The linearity range for LS and RS was found to be 30-150 µg/ml and 8-40 µg/ml, respectively. The recovery studies were performed at three different levels and the average results were found to be in the range of 99.14-100.64 % for LS and 99.47-100.46% for RS. The developed method was found to be precise when subjected to inter day and intraday precision studies. The robustness of the developed method was established by making deliberate changes in the critical parameters. The method was successfully applied for the estimation of LS and RS from their tablet dosage form. The developed RP-HPLC method can be successfully applied for the quality control and routine analysis of LS and RS from their combined formulations.
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